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C6344

Sigma-Aldrich

Monoclonal Anti-Connexin-32 antibody produced in mouse

clone CXN-32, ascites fluid, buffered aqueous solution

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MDL number:
MFCD00803531
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CXN-32, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

contains

15 mM sodium azide

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using a mouse whole brain extract

isotype

IgG1

UniProt accession no.

P08034

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GJB1(2705) , GJB2(2706)
mouse ... Gjb1(14618)
rat ... Gja1(24392) , Gjb1(29584)

Related Categories

General description

Monoclonal Anti-Connexin-32 (mouse IgG1 isotype) is derived from the CXN-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The 27 kDa connexin protein (Connexin-32, Cx32), belongs to the connexion family of proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types including peripheral and central nervous system.

Immunogen

synthetic connexin-32 peptide (amino acids 105-123).

Application

Monoclonal Anti-Connexin-32 antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • epifuorescent microscopy

Biochem/physiol Actions

Connexin-32 low expression levels is observed in common bile duct ligation (CBDL). In addition, a combination of myelin disruption and axonal degeneration has been shown to occur with Cx32 mutations in Charcot-Marie-Tooth disease (CMTX). Monoclonal antibodies reacting specifically with Cx32, may be used in diverse cellular and molecular approaches to the study of gap junctions and their properties, and to correlate their expression pattern with physiological functions or pathological conditions.
Connexins belong to the gap junction protein family. In humans, connexin is encoded by GJB1 (gap junction protein, β 1) gene. The gap junction proteins connexin32 (Cx32), Cx37, Cx40 and Cx43 are expressed in endothelial cells and regulate vascular functions involving inflammation. The endothelial Cx32 positively regulates angiogenesis by enhancing endothelial cell tube formation and cell migration. CX32 mutation is associated with cognitive impairment and a differential degree of peripheral nerve involvement.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Three-dimensional binding of epidermal growth factor peptides in colonic tissues produced from rotating bioreactor
Kaeffer B, et al.
In Vitro Cellular & Developmental Biology. Animal, 38(8), 436-439 (2002)
Connexins modulate autophagosome biogenesis
Bejarano E, et al.
Nature Cell Biology, 16(5), 401-401 (2014)
Role of the p38 MAP-kinase signaling pathway for Cx32 and claudin-1 in the rat liver
Kojima T, et al.
Cell communication & adhesion, 10(4-6), 437-443 (2003)
What?s the function of connexin 32 in the peripheral nervous system?
Bortolozzi M
Frontiers in Molecular Neuroscience, 11 (2018)
Yuksel Batir et al.
Archives of biochemistry and biophysics, 608, 8-19 (2016-07-06)
Alterations in gap junctions underlie the etiologies of syndromic deafness (KID) and Charcot-Marie Tooth disease (CMTX). Functional gap junctions are composed of connexin molecules with N-termini containing a flexible turn around G12, inserting the N-termini into the channel pore allowing
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