C6241
Charcoal, Dextran Coated
Synonym(s):
Dextran coated charcoal
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About This Item
form
powder
Quality Level
General description
Charcoal, Dextran Coated is made from acid washed charcoal powder and dextran, molecular weight 64-76kDa, combined in a 10:1 (w/w) ratio.
Application
Charcoal, Dextran Coated has been used:
- as a supplement in cell culture media to test the functioning of androgen receptor
- in the procedure of melatonin radioimmunoassay
- for the preparation of steroid-free human serum
- to reduce the creatinine level in untreated serum.
Biochem/physiol Actions
The use of dextran-coated charcoal makes the immunoassay of insulin in biological fluids simpler and more rapid. In theory, the charcoal coated with dextran will adsorb the free hormone and leave hormones that are bound to a carrier (or antibody). Dextran coated charcoal is used to strip hormones from serum instead of charcoal alone, because there is less loss of protein using dextran coated charcoal.
Other Notes
Dry preparation of dextran-treated charcoal
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Modern Methods of Steroid Analysis (2012)
Liang Shi et al.
Cell death & disease, 8(11), e3171-e3171 (2017-11-17)
Hepatocellular carcinoma (HCC) is a heterogeneous malignancy as a result of complex genetic and epigenetic alterations. HCC is characterized by a clear gender disparity for which there is lack of a clear mechanistic understanding. Androgen receptor (AR) is thought to
Multisteroid LC-MS/MS assay for glucocorticoids and androgens, and its application in Addison's disease.
Methlie P
Endocrine Connections (2013)
Shih-Hsing Leir et al.
Cell and tissue research, 381(2), 327-336 (2020-05-08)
Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms
Guangyi Jiang et al.
Aging, 12(12), 11466-11484 (2020-06-25)
Hepatocellular carcinoma (HCC) is a heterogeneous malignancy with gender-related differences in onset and course. Androgen receptor (AR), a male hormone receptor, is critical in the initiation and progression of HCC. The role of AR in HCC has been mechanistically characterized
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