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C6144

Sigma-Aldrich

Chlorophyll a

Powder

Synonym(s):

Pheophytin; universal photosynthetic pigment

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About This Item

Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
479-61-8
Molecular Weight:
893.49
Beilstein:
3586237
EC Number:
207-536-6
MDL number:
MFCD00079050
UNSPSC Code:
12171500
PubChem Substance ID:
24892839
NACRES:
NA.47

Product Name

Chlorophyll a, from Anacystis nidulans algae

biological source

Anacystis nidulans algae

Quality Level

200

form

powder

technique(s)

titration: suitable

impurities

chlorophyll b, free

color

blue to very dark blue

mp

150-153 °C

solubility

diethyl ether: 0.1 mg/10 mL

ε (extinction coefficient)

11.1-12.1 × 104 at 428-430 nm in diethyl ether
8.3-9.4 × 104 at 660-662 nm in diethyl ether

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

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Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used as a standard for the calibration of fluorometer.

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II. Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
Sealed ampule.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL5930
BCCM5739
BCCK5088
BCCJ6035
BCCH5004

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product C6144, Chlorophyll a, soluble in solvents other than diethyl ether?

    This product is soluble in ethanol (10 micrograms/mL). It is also freely soluble in acetone, benzene, and chloroform, but is not soluble in water. However, its spectral characteristics are most well characterized for solutions in diethyl ether.

  6. Can Product C6144, Chlorophyll a, be used as an analytical standard?

    We do not recommend using this product as a standard. The fill weights of 1 mg and 5 mg are minimum values and are not guaranteed to a specific precision. Also, the product is not traceable to a consensus standard reference material.

  7. What is the purity of Product C6144, Chlorophyll a, as determined by chromatographic analysis (e.g., by HPLC)?

    We do not determine the purity of Product No. C6144 by chromatographic means. We offer an HPLC tested Chlorophyll a (isolated from spinach) as Product no. C5753.

  8. How does chlorophyll a from blue green algae differ from that found in plants?

    A complete analysis of chlorophyll a between species would require extensive study. We can say that the CAS number of this product is the same as that for chlorophyll a from spinach, a typical green plant.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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