All Photos(1)
Synonym(s):
Calcium-activated neutral protease 1
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
Assay
≥95% (SDS-PAGE)
form
aqueous glycerol solution
specific activity
≥1000 U/mg
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CAPN1(823)
Application
Human calpain 1 has been used in a study to assess how the crystal structures of human calpains 1 and 9 imply diverse mechanisms of action and auto-inhibition. Human calpain 1 has also been used in a study to investigate the synthesis, biological evaluation and molecular modelling of N-heterocyclic dipeptide aldehydes as selective calpain inhibitors.
Biochem/physiol Actions
Caplain 1 is a neutral calcium-dependent cysteine protease containing the EF-hand motif. The protease consists of two subunits; the larger subunit has four domains that are homologous with papain and calmodulin. The smaller subunit has one domain that shares homology with calmodulin. It is activated by micromolar levels of calcium and hence, it is also called as micro-calpain. Its activation leads to cellular protein degradation, neuronal cell degeneration, and autoimmune demyelinating diseases such as multiple sclerosis.
Cytosolic protease with involvement in cytoskeletal remodeling, autophagy, and apoptosis as an upstream regulator.
Physical properties
Calpain 1 is a heterodimeric calcium-activated thiol-protease.
Unit Definition
One unit will hydrolyze 1 picomole Suc-LLVY-AMC per minute at 25 deg C.
Physical form
Solution in 20 mM Imidazole-HCl, 5 mM β-Mercaptoethanol, 1 mM EDTA, 1 mM EGTA, and 30% glycerol.
inhibitor
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
高风险级别生物产品--人源产品
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Tarun Tyagi et al.
Blood, 123(8), 1250-1260 (2013-12-04)
Oxygen-compromised environments, such as high altitude, air travel, and sports, and pathological conditions, such as solid tumors, have been suggested to be prothrombotic. Despite the indispensable role of platelets in thrombus formation, the studies linking hypoxia, platelet reactivity, and thrombus
Nicholas B Woodall et al.
Nature structural & molecular biology, 28(9), 762-770 (2021-09-15)
Kinases play central roles in signaling cascades, relaying information from the outside to the inside of mammalian cells. De novo designed protein switches capable of interfacing with tyrosine kinase signaling pathways would open new avenues for controlling cellular behavior, but
Xuan Zhang et al.
PloS one, 7(2), e31458-e31458 (2012-02-23)
Hepatitis B virus X protein (HBx) plays an important role in the development of hepatocellular carcinoma (HCC). However, the mechanism remains unclear. Recently, we have reported that HBx promotes hepatoma cell migration through the upregulation of calpain small subunit 1
M K Squier et al.
Journal of immunology (Baltimore, Md. : 1950), 158(8), 3690-3697 (1997-04-15)
Apoptosis is the common phenotype of programmed or physiologic cell death, the process used to remove excess or defunct cells during normal tissue maintenance. One of the most studied cell types with respect to apoptosis is the immature T cell
Rebecca M Kandell et al.
ACS nano, 15(12), 20504-20516 (2021-12-07)
Traumatic brain injury (TBI) is a critical public health concern and major contributor to death and long-term disability. After the initial trauma, a sustained secondary injury involving a complex continuum of pathophysiology unfolds, ultimately leading to the destruction of nervous
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