C5890
Casein from bovine milk
suitable for substrate for protein kinase (after dephosphorylation), purified powder
About This Item
biological source
bovine milk
Quality Level
form
purified powder
technique(s)
activity assay: suitable
electrophoresis: suitable
immunocytochemistry: suitable
mp
280 °C (dec.) (lit.)
suitability
suitable for substrate for protein kinase (after dephosphorylation)
InChI
1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)
InChI key
BECPQYXYKAMYBN-UHFFFAOYSA-N
Gene Information
bovine ... CSN1S1(282208) , CSN1S2(282209) , CSN2(281099) , CSN3(281728)
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General description
Application
- to prepare casein hydrolysates
- as a calibrating substance in biuret colorimetric method
- as a supplement in nutrient agar for the determination of protease activity
- to prepare protein-enriched diet for independent replicate lines of D. melanogaster
Biochem/physiol Actions
WGK
WGK 1
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the composition of Product C5890, Casein from bovine milk?
The approximate casein composition of milk in grams per Liter is: alpha-s1 (12-15); alpha-s2 (3-4); beta (9-11); and kappa (2-4).
What is the molecular weight of the casein subunits in Product C5890, Casein from bovine milk?
The alpha-s1 = 22-23.7 kDa, alpha-s2 = 25 kDa, beta = 24 kDa and kappa = 19 kDa.
What is Product C5890, Casein from bovine milk, soluble in?
Casein is not water soluble. It can be suspended in 1 M sodium hydroxide at a concentration of 50 mg/mL, yielding a turbid, faint yellow solution.
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Chromatograms
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