C5838
Monoclonal Anti-Actin–Cy3 antibody produced in mouse
clone AC-40, purified immunoglobulin, buffered aqueous solution
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Synonym(s):
Monoclonal Anti-Actin
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-40, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
technique(s)
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.
Immunogen
synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone
Application
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
Biochem/physiol Actions
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Legal Information
Distributed under license from Amersham Biosciences Limited.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Erin Faught et al.
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Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
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