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Merck
CN

C5753

Chlorophyll a

from spinach

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About This Item

Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
Molecular Weight:
893.49
UNSPSC Code:
12171506
NACRES:
NA.47
PubChem Substance ID:
EC Number:
207-536-6
Beilstein/REAXYS Number:
3586237
MDL number:
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Product Name

Chlorophyll a, from spinach

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

biological source

spinach

assay

≥85% (HPLC)

form

powder

impurities

chlorophyll b, essentially free

ε (extinction coefficient)

10.6-11.8 × 104 at 428 nm in diethyl ether (vs. 11.17 × 104, lit.)
8.2-9.1 × 104 at 662 nm in diethyl ether (vs. 8.63 × 104, lit.)

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used:
  • to study the variation in carotenoids composition with changing colors and maturation in soybean seeds
  • to examine the use of chlorophyll distribution maps obtained from hyperspectral imaging for the non-destructive diagnosis of Nitrogen deficiency
  • as a standard to determine the concentration of the analyte by fluorescence spectrophotometric technique in the water sample collected from the yellow river
  • as a standard to quantify the amount of chlorophyllous pigments gathered from algae by high performance liquid chromatographic (HPLC) technique
  • in the identification of pheophytin a from environmentally friendly cultivated green peppers
  • to sense different nitric oxide (NO) species

Biochem/physiol Actions

Chlorophyll a binds to the protein molecules CP47 and CP43, forming the inner antenna of the reaction center of Photosystem II. In photosystem I, the binding sites of chlorophyll a includes the polypeptides spread across the membranes. Scarcity in formation of chlorophyll and a low chlorophyll density can lead to deficiency of nitrogen in plants leaves.

General description

Chlorophyll a is an important light-harvesting, green or blue color pigment present in the photosynthetic membranes of the chloroplasts in plant cells. It functions as an antenna pigment in photosystems I and II.

Packaging

Sealed ampule.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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  1. Is my ampule intact? How can I tell?

    If the meniscus "bubble" is not broken, then the ampule is intact and usable. To test the meniscus, use a glass Pasteur pipet and gently touch the bubble. If the glass pops and breaks, then the ampule was sealed. If it goes straight through to the bottom of the ampule with no resistance, then the seal was broken during transit.

The determination of algal chlorophyllous pigments by high performance liquid chromatography and spectrophotometry
Sartory DP
Water Research, 19(5), 605-610 (1985)
Carotenoid components in soybean seeds varying with seed color and maturation stage
Monma M, et al.
Bioscience, Biotechnology, and Biochemistry, 58(5), 926-930 (1994)
Lockhart and Wiseman?s Crop Husbandry Including Grassland, 985(2), 101e113-101e113 (2014)
Photobiology: The Science of Life and Light (2007)
Nondestructive diagnostics of nitrogen deficiency by cucumber leaf chlorophyll distribution map based on near infrared hyperspectral imaging
Ji-Yong S, et al.
Sci. Hortic., 138, 190-197 (2012)

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