C5348
Anti-Corticotropin Releasing Factor antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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Synonym(s):
CRF Antibody - Anti-Corticotropin Releasing Factor antibody produced in rabbit, Crf Antibody, Anti-CRF
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
frog, canine, sheep, rat, human, mouse, pig, bovine
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue
microarray: suitable
radioimmunoassay: 1:10,000 using 2-125I-His12-CRF, human
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CRH(1392)
mouse ... Crh(12918)
rat ... Crh(81648)
General description
Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN).
Specificity
The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.
Immunogen
synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.
Application
Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry (1:2000) and radioimmunoassay.
Biochem/physiol Actions
Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-adrenal gland axis, and autonomic and behavioral responses to stress. CRF effects include changes in motor and cognitive behavior, appetite control and modulation of the immune system. CRF is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. The effects of CRF are mediated through CRF receptor subtypes CRFR1 and CRFR2 (splice variants). CRF binds with a high affinity to CRF-binding protein (CRF-BP), which inactivates CRF, while ligands that dissociate CRF from CRF-BP exhibit cognition-enhancing properties. CRF overproduction has been implicated in anorexia and depression and may lead to Cushing′s syndrome. CRF levels are correlated with Alzheimer′s Disease (AD) pathogenesis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Jorianne Boers et al.
BMJ case reports, 12(11) (2019-11-23)
In this case report, we highlight four different cases of ectopic adrenocorticotropic hormone (ACTH) secretion with different pitfalls in the diagnostic workup. Ectopic ACTH secretion (EAS) is an uncommon cause of Cushing's syndrome that accompanies a variety of tumours. It
Roeland Buckinx et al.
Frontiers in neuroscience, 5, 54-54 (2011-05-05)
Corticotrophin-releasing factor (CRF) is mainly known for its role in the stress response in the hypothalamic-pituitary-adrenal axis. However, increasing evidence has revealed that CRF receptor signaling has additional peripheral effects. For instance, activation of CRF receptors in the gastrointestinal tract
Sooyoung Chung et al.
Endocrinology, 146(7), 3202-3210 (2005-04-02)
It is well established that stress in early life can alter the activity of the hypothalamus-pituitary-adrenal (HPA) axis, but most studies to date have focused on HPA reactivity in response to a single acute stress. The present study addressed whether
Van A Ortega et al.
Frontiers in neuroscience, 7, 196-196 (2013-11-07)
Corticotropin-releasing factor (CRF), urotensin I (UI) and serotonin (5-HT) are generally recognized as key regulators of the anorexigenic stress response in vertebrates, yet the proximal effects and potential interactions of these central messengers on food intake in salmonids are not
M P Stenzel-Poore et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 14(5 Pt 1), 2579-2584 (1994-05-01)
Corticotropin-releasing factor (CRF) is released in response to various stressors and regulates adrenocorticotropin secretion and glucocorticoid production. In addition to its endocrine functions, CRF acts as a neuromodulator in extra-hypothalamic systems and has been shown to play a role in
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