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C50120

Sigma-Aldrich

CM Sephadex® C-50

Synonym(s):

CM Sephadex®, Carboxymethyl Sephadex®

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About This Item

CAS Number:
9047-08-9
MDL number:
MFCD00132776
UNSPSC Code:
47101511
NACRES:
NA.56

form

powder

Quality Level

200

technique(s)

activity assay: suitable

bead size

40-125 μm (dry)

pore size

~200,000 Da exclusion limit

operating pH

6-10

capacity

4.0-5.0 meq/g ion exchange capacity

compatibility

mode of use weak cation exchange chromatography

General description

C50120-100G′s updated product number is GE17-0220-01

Application

Sephadex C-50 is used for cation exchange media, separation media, resins, ion exchange chromatography, protein chromatography and affinity chromatography. Sephadex® C-50 has been used to study biochemical characteristics of cobra venom and effects of vitamins on lactoperoxidase enzyme activity.

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000268986
0000268985
0000237724
0000237724
0000268985

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What molecular weight range is CM Sephadex® C-50, Product C50120, suitable for?

    Sephadex C-50 is suitable for larger biomolecules such as proteins in the molecular weight range of 30 to 100 kDa.

  6. How do I prepare CM Sephadex® C-50, Product C50120?

    Weigh out the required amount of dry powder and suspend it in binding buffer. In general, 1 gram of dry powder CM Sephadex C-50 will give about 30 mL final volume, when swollen in saline buffer.  Swelling should be done at the pH at which the resin is going to be used. Complete swelling takes 1-2 days at room temperature, or 2 hours at 100°C over boiling water.

  7. How do I regenerate CM Sephadex® C-50, Product C50120?

    Depending on the nature of the sample, regeneration is normally preformed by washing with a high ionic strength buffer (1-2 M NaCl) and/or increasing pH, followed by re-equilibrating in binding buffer.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Human liver acid phosphatases: purification and properties of a low-molecular-weight isoenzyme.
E M Taga et al.
Archives of biochemistry and biophysics, 214(2), 505-515 (1982-04-01)
N J Abbott et al.
Journal of cell science, 103 ( Pt 1), 23-37 (1992-09-01)
Primary culture of rat brain endothelial cells is described, based on the method of C. C. W. Hughes and P. L. Lantos. The cells have been characterised using morphological and immunocytochemical techniques, and systematic studies undertaken to determine the optimal
Purification and properties of an endoglucanase isolated from the cell walls of <I>Zea mays</I> seedlings.
Hatfield, R., and Nevis, D.J.
Carbohydrate Research, 148(2), 265-278 (1986)
C Chandra Sekhar et al.
Journal of biosciences, 36(2), 355-361 (2011-06-10)
A fibrinogenolytic toxin of molecular weight 6.5 kDa has been purified from the venom of Indian monocled cobra (Naja kaouthia) by repeated cation exchange chromatography on CM-sephadex C-50. The purified toxin did not show any phospholipase activity but was mildly
inetic studies on 1:1 electron-transfer reactions involving blue copper proteins. 15. The reactivity of <I>Anabaena variabilis</I> plastocyanin with inorganic complexes and related NMR studies.
Jackman, M.P., et al.
Journal of the American Chemical Society, 109(21), 6443-6449 (1987)
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