Skip to Content
Merck
CN
All Photos(2)

Key Documents

View All Documentation

Safety Information

C4482

Sigma-Aldrich

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Synonym(s):

Yeast cell lysis reagent

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116134
NACRES:
NA.56

Quality Level

200

form

solution

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Features and Benefits

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

监管及禁止进口产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000373971
0000373970
0000342070
0000342932
0000327291

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    If cell growth conditions are not optimal, then glass beads can be used to increase yields.

  4. Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?

    For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent.  Addition of DTT significantly improves total protein yield.

  5. Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?

    No.  For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.

  6. What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?

    The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.

  7. What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)

Articles

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Related Content

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service