C4405
Choline Oxidase from Arthrobacter globiformis
lyophilized powder, 8-20 units/mg solid
Synonym(s):
Choline: oxygen 1-oxidoreductase
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About This Item
form
lyophilized powder
Quality Level
specific activity
8-20 units/mg solid
mol wt
71 kDa by SDS-PAGE
83 kDa by gel filtration
storage temp.
−20°C
Related Categories
General description
Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.
Application
Choline Oxidase from Arthrobacter globiformis has been used:
- for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
- in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
- in enzyme coating for the fully-assembled recording device for electrochemical measurements
Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.
Biochem/physiol Actions
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.
Unit Definition
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Choline import into chloroplasts limits glycine betaine synthesis in tobacco: analysis of plants engineered with a chloroplastic or a cytosolic pathway
Nuccio ML, et al.
Metabolic engineering, 2(4), 300-311 (2000)
A multi-site array for combined local electrochemistry and electrophysiology in the non-human primate brain
Disney AA, et al.
Journal of Neuroscience Methods, 255, 29-37 (2015)
S Ikuta et al.
Journal of biochemistry, 82(6), 1741-1749 (1977-12-01)
Choline oxidase was purified from the cells of Arthrobacter globiformis by fractionations with acetone and ammonium sulfate, and column chromatographies on DEAE-cellulose and on Sephadex G-200. The purified enzyme preparation appeared homogeneous on disc gel electrophoresis. The enzyme was a
J L Lima et al.
The Analyst, 125(7), 1281-1284 (2000-09-14)
The development of a FIA system for the determination of total choline content in several types of milk is described. The samples were submitted to hydrochloric acid digestion before injection into the system and passed through an enzymatic reactor containing
Fan Fan et al.
Journal of the American Chemical Society, 127(7), 2067-2074 (2005-02-17)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, with betaine aldehyde as an intermediate. In this study, primary deuterium and solvent kinetic isotope effects have been used to elucidate the mechanism for substrate oxidation by choline oxidase
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