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Key Documents

Safety Information

C-41210

Sigma-Aldrich

DetachKit

3 x 125 ml

Synonym(s):

Cell separation kit

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250 ML
CN¥1,869.36

CN¥1,869.36

Available to ship onApril 16, 2025Details

Request a Bulk Order
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250 ML
CN¥1,869.36

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

CN¥1,869.36

Available to ship onApril 16, 2025Details

Request a Bulk Order

packaging

pkg of 3 × 125 ml

technique(s)

cell culture | mammalian: suitable

shipped in

cooler

storage temp.

−20°C

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application(s)

environmental
food and beverages

application(s)

environmental
food and beverages

application(s)

food and beverages

application(s)

environmental
food and beverages

material

UHMW PE frit, polypropylene tube

material

UHMW PE frit, polypropylene tube

material

PTFE frit, polypropylene tube

material

polypropylene tube

agency

suitable for DIN 38407-42

agency

suitable for DIN 38407-42, suitable for EPA 1633, suitable for EPA 533, suitable for EPA 537.1, suitable for EPA ACB B21-02, suitable for EPA ACB B23-05b, suitable for EPA OTM-45, suitable for GB 31604.35-2016, suitable for ISO 21675 2019, suitable for ISO 25101, suitable for ISO/CEN 15968-2010

agency

-

agency

-

reg. compliance

suitable for FDA C-010.02

reg. compliance

suitable for FDA C-010.02

reg. compliance

-

reg. compliance

-

product line

Visiprep™

product line

Visiprep™

product line

Visiprep™

product line

-

General description

DetachKit-125

Application

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Components

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Quality

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

Regulatory Information

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