C3921
Creatinase from Pseudomonas sp.
recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein
Synonym(s):
Creatine Amidinohydrolase
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
10-15 units/mg protein
mol wt
45 kDa
composition
Protein, ~70% biuret
storage temp.
2-8°C
Application
Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.
Biochem/physiol Actions
Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.
Unit Definition
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Jun Chen et al.
Toxicological sciences : an official journal of the Society of Toxicology, 108(1), 81-89 (2009-01-20)
Hepatotoxic microcystins (MCs) are the most commonly reported cyanotoxins in eutrophic freshwaters. In 1996, human intoxications by MCs caused deaths of 76 patients at Caruaru dialysis centers in Brazil. So far, there have been no direct evidences of MC occurrence
Elizabeth F Daher et al.
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, 12(4), 329-332 (2008-11-26)
Visceral leishmaniasis is an endemic disease caused by various species of Leishmania. We made a retrospective study of 57 consecutive patients with visceral leishmaniasis in Brazil. Patients with visceral leishmaniasis were identified using the registries of the São José Infectious
M-L Lee
Pediatric cardiology, 26(6), 792-796 (2005-08-06)
I report on a 3-month-old infant with pulmonary atresia-intact ventricular septum and ventriculocoronary communication (VCC) who underwent percutaneous radiofrequency valvulotomy and valvuloplasty (RFVV). The patient's cardiac troponin-I, creatine kinase (CK), and myocardial fraction of (CK-MB) were elevated before RFVV and
N A Siddiqi et al.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia, 17(2), 203-207 (2006-08-15)
Polyoma virus nephropathy (BK virus) is being recognized as an important cause of graft failure. It is usually confused with acute rejection. No cases have been reported from the kingdom of Saudi Arabia. We report a case of a Saudi
Takashi Nakamura et al.
Bioscience, biotechnology, and biochemistry, 70(10), 2363-2370 (2006-10-13)
Aspergillus nidulans possessed 16 putative amylolytic genes consisting of 7 alpha-glucosidase (agdA-F), 7 alpha-amylase (amyA-F), and 2 glucoamylase (glaA and B) genes on the genome. Among them, the agdA, agdB, agdE, agdF, amyA, amyB, amyF, and glaB genes were induced
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