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C3868

Sigma-Aldrich

Anti-Calsequestrin-2 antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CASQ2, Anti-fast-twitch, cardiac muscle Calsequestrin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, human

species reactivity (predicted by homology)

mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2
western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)

UniProt accession no.

O14958

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASQ2(845)
mouse ... Casq2(12373)
rat ... Casq2(29209)

Related Categories

General description

Calsequestrin is a major Ca2+ binding protein in cardiac and skeletal muscle.
Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

Immunogen

synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.

Application

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.
Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

Biochem/physiol Actions

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the sarcoplasmic reticulum (SR).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Absence of calsequestrin 2 causes severe forms of catecholaminergic polymorphic ventricular tachycardia
Postma A V, et al.
Circulation Research, 91(8), e21-e26 (2002)
Calsequestrin 2 and arrhythmias
Faggioni M and Knollmann B C.
American Journal of Physiology. Heart and Circulatory Physiology, 302(6), H1250-H1260 (2011)
Kathy Ye Morgan et al.
Tissue engineering. Part A, 20(11-12), 1654-1667 (2014-01-15)
Electrical and mechanical stimulation have both been used extensively to improve the function of cardiac engineered tissue as each of these stimuli is present in the physical environment during normal development in vivo. However, to date, there has been no
Kathy Ye Morgan et al.
Journal of tissue engineering and regenerative medicine, 11(2), 342-353 (2014-06-12)
Mechanical stimulation has been used extensively to improve the function of cardiac engineered tissue, as it mimics the physical environment in which the tissue is situated during normal development. However, previous mechanical stimulation has been carried out under a constant
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio
Furlan S, et al.
Journal of Muscle Research and Cell Motility, 37(1-2), 27-39 (2016)
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