C3657
Human Collagen Type V
from human placenta, powder, suitable for detection assays
Sign Into View Organizational & Contract Pricing
All Photos(1)
Recommended Products
product name
Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder
biological source
human placenta
Quality Level
form
powder
impurities
HIV, hepatitis B and hepatitis C, none detected
solubility
aqueous acid: soluble
UniProt accession no.
application(s)
detection
storage temp.
2-8°C
Gene Information
human ... COL5A1(1289)
Looking for similar products? Visit Product Comparison Guide
Components
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.
Caution
This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.
Preparation Note
This product was prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
Analysis Note
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Other Notes
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The serotype a-EmaA adhesin of Aggregatibacter actinomycetemcomitans does not require O-PS synthesis for collagen binding activity.
Tang-Siegel, et al.
Microbiology (Reading, England), 168 (2023)
Gaoyan Tang et al.
Microbiology (Reading, England), 153(Pt 8), 2447-2457 (2007-07-31)
Adhesion of Aggregatibacter actinomycetemcomitans to extracellular matrix proteins is mediated by antennae-like surface structures composed of EmaA oligomers. EmaA is an outer-membrane protein orthologous to the autotransporter YadA, a virulence determinant of Yersinia. emaA was present in the 27 strains
Identification of autoantigens and their potential post-translational modification in EGPA and severe eosinophilic asthma.
Esposito, et al.
Frontiers in Immunology, 14, 1164941-1164941 (2023)
Gaoyan G Tang-Siegel et al.
Journal of bacteriology, 204(12), e0021522-e0021522 (2022-12-01)
The human oral pathobiont Aggregatibacter actinomycetemcomitans expresses multiple virulence factors, including the trimeric, extracellular matrix protein adhesin A (EmaA). The posttranslational modification of EmaA is proposed to be dependent on the sugars and enzymes associated with O-polysaccharide (O-PS) synthesis of
Sarah Finke et al.
Cell surface (Amsterdam, Netherlands), 5, 100032-100032 (2020-08-18)
Cyclic diguanylate (c-di-GMP) signalling affects several cellular processes in Bacillus cereus group bacteria including biofilm formation and motility, and CdgF was previously identified as a diguanylate cyclase promoting biofilm formation in B. thuringiensis. C-di-GMP can exert its function as a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service