C3640
Carbonic Anhydrase Isozyme II from bovine erythrocytes
lyophilized powder, ≥2,000 W-A units/mg protein
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form
lyophilized powder
Quality Level
specific activity
≥2,000 W-A units/mg protein
pI
~5.4
storage temp.
−20°C
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Application
Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.
Biochem/physiol Actions
Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in pathologies such as glaucoma, epilepsy and Parkinson′s disease.
Unit Definition
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Todd S Davidson et al.
The American journal of pathology, 166(3), 729-736 (2005-03-04)
Autoimmune pancreatitis (AIP), a recently defined disease of unknown etiology, is characterized by inflammatory infiltrates in the pancreas with conspicuous involvement of the ducts. The disease clinically manifests in humans as epigastric pain, weight loss, and jaundice. This report describes
Daumantas Matulis et al.
Biochemistry, 44(13), 5258-5266 (2005-03-30)
ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding
Romualdo Talento et al.
Ultrastructural pathology, 37(1), 70-76 (2013-02-07)
Despite progress in the classification of renal cell carcinomas (RCC), a subset of these carcinomas remains unclassified (RCC-U). Patients with RCC-U usually present at a late stage and have a poor prognosis. Several studies have attempted to extract new classifications
Wolfgang Maret
Advances in nutrition (Bethesda, Md.), 4(1), 82-91 (2013-01-16)
The nutritional essentiality of zinc for the growth of living organisms had been recognized long before zinc biochemistry began with the discovery of zinc in carbonic anhydrase in 1939. Painstaking analytical work then demonstrated the presence of zinc as a
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
Protocols
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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