C3117
Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse
~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution
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Synonym(s):
Monoclonal Anti-Cy3/Cy5
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CY-96, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.
Specificity
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
Immunogen
mixture of proteins labeled with Cy3/Cy5.
Application
Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- dot blot assay
- protein microarrays
- immunofluorescenc
Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.
Biochem/physiol Actions
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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