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C3042

Sigma-Aldrich

Corticotropin Releasing Factor human, rat

≥95% (HPLC), powder

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Synonym(s):
CRF, CRH
Empirical Formula (Hill Notation):
C208H344N60O63S2
CAS Number:
Molecular Weight:
4757.45
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human
rat

Quality Level

Assay

≥95% (HPLC)

form

powder

mol wt

4757.45

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

technique(s)

activity assay: suitable

solubility

water: 1.00-1.04 mg/mL, colorless

UniProt accession no.

storage temp.

−20°C

InChI key

VXFVFWFSJFSXHN-FAUHKOHMSA-N

Gene Information

human ... CRH(1392)

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Amino Acid Sequence

Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2

General description

The corticotropin-releasing factor is a 41-amino-acid polypeptide. It is expressed in neurons, the hypothalamus, and the amygdala.

Research area: Neuroscience

Application

Corticotropin Releasing Factor human, rat has been used:
  • as a component in Dulbecco′s modified eagle medium (DMEM) for incubating lung explants
  • as a component in serum-free complete medium for isolation and culturing of fetal distal lung epithelial cells
  • in the synthesis of the corticotropin-releasing factor(CRF)-TAMRA 1 mixture
  • in chronic intracerebroventicular administration, to study its effect on hypoxia in hippocampus

Biochem/physiol Actions

CRF is a hypothalamic peptide that releases ACTH and endorphin from the anterior pituitary. It is also a neurotransmitter in the central nervous system, and is involved in autonomic and endocrine responses to stress.
Corticotropin-releasing factor modulates stress responses. It facilitates stress-stimulated activation of the hypothalamic−pituitary−adrenal system.Corticotropin Releasing Factor/hormone (CRF/H) at higher levels has been observed in stress-related physiological and behavioral disorders. It has been involved in anxiety and depression disorders. Under stress conditions, the release of CRH activates the corticotrophin-releasing hormone receptor type I (CRHR1) in the anterior pituitary for the release of adrenocorticotropic hormone (ACTH) that further stimulates the production/secretion of cortisol/corticosterone.

Other Notes

Lyophilized from 0.1% TFA in H2O

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Corticotropin Releasing Factor human, rat (C3042)?

    Based on our quality control testing, Corticotropin Releasing Factor human, rat (C3042) is soluble in water at a concentration of 1 mg/ml.

  6. What is the solution stability of Corticotropin Releasing Factor human, rat (C3042)?

    Sigma has not tested Corticotropin Releasing Factor human, rat (C3042) for solution stability or for stability in culture.  The following article looks at the stability of corticotropin releasing factor: Acta Med Okayama. 1985 Jun;39(3):199-206.   

  7. What is the difference between the peptide content and the purity of Corticotropin Releasing Factor human, rat (C3042)?

    The peptide content of Corticotropin Releasing Factor human, rat (C3042) is determined by amino acid analysis (AAA) and distinguishes how much of the product is actually peptide.  The purity of Corticotropin Releasing Factor human, rat (C3042) is determined by HPLC and distinguishes how much of the peptide found is Corticotropin Releasing Factor human, rat (C3042).

  8. What is the counter ion in Corticotropin Releasing Factor human, rat (C3042)?

    Corticotropin Releasing Factor human, rat (C3042) is lyophilized from 0.1% TFA in water.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Frontiers in Psychiatry, 3-28 (2014)
Transcriptional regulation of hypothalamic corticotropin-releasing factor gene
Vitamins & Hormones, 82, 301-317 (2010)
Expression of genes related to the hypothalamic-pituitary-adrenal axis in murine fetal lungs in late gestation
Simard M, et al.
Reproductive Biology and Endocrinology, 8(1), 134-134 (2010)
Salt appetite
Vitamins & Hormones (2017)
A Slominski
The British journal of dermatology, 160(2), 229-232 (2009-02-04)
Corticotropin-releasing hormone (CRH; previously known as corticotropin-releasing factor) is the central regulator of the hypothalamic-pituitary-adrenal (HPA) axis, which is the main organizer of the body’s response to stress.– Stress induces the hypothalamic production and release of CRH, which then causes

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