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C2885

Sigma-Aldrich

CHES

≥99.0% (titration)

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Synonym(s):
2-(Cyclohexylamino)ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
103-47-9
Molecular Weight:
207.29
Beilstein:
2967601
EC Number:
203-115-6
MDL number:
MFCD00003835
UNSPSC Code:
12161700
PubChem Substance ID:
24892541
NACRES:
NA.25

Quality Level

200

Assay

≥99.0% (titration)

form

crystalline

pH

3.0-5.0 (103.6 g/L)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

water: 0.6 g/25 mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:
  • as a buffer in mannanase activity assay of antarctic fungal isolates
  • as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
  • as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods
Gujska E and Kuncewicz A
European Food Research and Technology, 221(1-2), 208-213 (2005)
Jun Yang et al.
Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to
R C Bray et al.
Biochemistry, 39(37), 11258-11269 (2000-09-14)
Much is unknown concerning the role of thiolate ligands of molybdenum in molybdopterin enzymes. It has been suggested that thiolate dissociation from molybdenum is part of the catalytic mechanism of bis-molybdopterin enzymes of the dimethyl sulfoxide reductase (DMSOR) family. For
Hao Zhong et al.
The Analyst, 136(21), 4486-4491 (2011-09-29)
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids naringenin, hesperetin, naringin and hesperidin. Separation conditions, including the background electrolyte pH and concentration, the length and choice of
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