C2747
Anti-CPSF3 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-CPSF73, Anti-Cleavage and Polyadenylation Specificity Factor 3, Anti-YSH1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
human
technique(s)
western blot: 1:250-1:500 using K-562 lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CPSF3(51692)
General description
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. This gene is a member of the metallo-β-lactamase family and is referred as CPSF73 and CPSF-73. CPSF73 is a 73kDa subunit and is the pre-mRNA 3′-end-processing endonuclease.
Application
Anti-CPSF3 (C-terminal) antibody produced in rabbit is suitable for western blotting at a dilution of 1:250-1:500 using K-562 lysate.
Biochem/physiol Actions
CPSF3 is induced by interaction between CSR1 and CPSF3 and is translocated from the nucleus to the cytoplasm, resulting in inhibition of polyadenylation. Down-regulation of CPSF3 using small interfering RNA induces cell death. CPSF73 mediates cleavage coupled to polyadenylation and histone pre-mRNA processing. HIV-1 Tat protein interacts with CPSF-73 and counteracts its repressive activity on the HIV-1 LTR promoter. During the cleavage and polyadenylation, CPSF plays a central role in processing the reaction.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Z-H Zhu et al.
Oncogene, 28(1), 41-51 (2008-09-23)
CSR1 (cellular stress response 1), a newly characterized tumor-suppressor gene, undergoes hypermethylation in over 30% of prostate cancers. Re-expression of CSR1 inhibits cell growth and induces cell death, but the mechanism by which CSR1 suppresses tumor growth is not clear.
Laureano de la Vega et al.
Journal of molecular biology, 372(2), 317-330 (2007-08-03)
Gene expression in eukaryotes requires the post-transcriptional cleavage of mRNA precursors into mature mRNAs. The cleavage and polyadenylation specificity factor (CPSF) is critical for this process and its 73 kDa subunit (CPSF-73) mediates cleavage coupled to polyadenylation and histone pre-mRNA
A Jenny et al.
Science (New York, N.Y.), 274(5292), 1514-1517 (1996-11-29)
The 3' ends of most eukaryotic messenger RNAs are generated by endonucleolytic cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF) plays a central role in both steps of the processing reaction. Here, the cloning of the
Corey R Mandel et al.
Nature, 444(7121), 953-956 (2006-11-28)
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses
Lee Davidson et al.
Genes & development, 28(4), 342-356 (2014-01-31)
3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how
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