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C-27437

Sigma-Aldrich

Preadipocyte Differentiation Medium

Kit including Basal Medium and SupplementPack, 500 ml

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UNSPSC Code:
12352207
NACRES:
NA.81

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Preadipocyte Differentiation Medium Kit

Application

Preadipocyte Differentiation Medium is a serum-free medium, which induces the differentiation of preadipocytes into mature adipocytes. The mature adipocytes can be cultivated using the Adipocyte Nutrition Medium. The media are optimized for primary human cells, but can also be used for murine preadipocytes.Recommended for:Human White Preadipocytes (HWP)

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:d-Biotin: 8 µg / mlInsulin (recombinant human): 0.5 µg / mlDexamethasone: 400 ng / mlIBMX: 44 µg / mlL-Thyroxine: 9 ng / mlCiglitazone: 3 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Subculture Routine

Click here for more information.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 3

Regulatory Information

新产品

Certificates of Analysis (COA)

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Articles

Primary Human Preadipocytes and Preadipocyte/Adipocyte Growth & Differentiation Media

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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