C2522
Carbonic Anhydrase Isozyme II from bovine erythrocytes
lyophilized powder, ≥3,000 W-A units/mg protein
Synonym(s):
Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
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About This Item
biological source
bovine erythrocytes
Quality Level
form
lyophilized powder
specific activity
≥3,000 W-A units/mg protein
mol wt
30 kDa
pI
~5.9
storage temp.
−20°C
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Application
Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.
Biochem/physiol Actions
Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.
Unit Definition
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rui Hou et al.
ACS omega, 8(21), 19057-19071 (2023-06-05)
DNA-encoded library (DEL) is a powerful ligand discovery technology that has been widely adopted in the pharmaceutical industry. DEL selections are typically performed with a purified protein target immobilized on a matrix or in solution phase. Recently, DELs have also
Maria M Garcia-Alai et al.
Nature communications, 9(1), 328-328 (2018-01-25)
In clathrin-mediated endocytosis, adapter proteins assemble together with clathrin through interactions with specific lipids on the plasma membrane. However, the precise mechanism of adapter protein assembly at the cell membrane is still unknown. Here, we show that the membrane-proximal domains
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Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a
Arne Schön et al.
Analytical biochemistry, 488, 45-50 (2015-08-05)
Protein aggregation is a major issue affecting the long-term stability of protein preparations. Proteins exist in equilibrium between the native and denatured or partially denatured conformations. Often denatured or partially denatured conformations are prone to aggregate because they expose to
Gérard Liger-Belair et al.
ACS omega, 8(25), 22844-22853 (2023-07-03)
When it comes to champagne tasting, dissolved CO2 is a key compound responsible for the very much sought-after effervescence in glasses. Nevertheless, the slow decrease of dissolved CO2 during prolonged aging of the most prestigious cuvees raises the issue of
Protocols
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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