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Merck
CN

C2124

Bovine Collagen Type I

6 mg/mL, sterile-filtered, (suitable for cell culture and for 3D matrix formation), BioReagent

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00130825

Key Documents

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Product Name

Collagen solution from bovine skin, 6 mg/mL, sterile-filtered, (suitable for cell culture and for 3D matrix formation), BioReagent

biological source

bovine skin

sterility

sterile-filtered

product line

BioReagent

form

liquid

packaging

pkg of 50 mL

concentration

6 mg/mL

technique(s)

cell culture | mammalian: suitable using and for 3D matrix formation.

surface coverage

6‑10 μg/cm2

binding specificity

Peptide Source: Collagen

Peptide Source: Elastin

Peptide Source: Fibronectin

foreign activity

endotoxin ≤1.0 μmole/min-mg protein

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Application

Collagen solution from bovine skin has been used as a substrate in cell adhesion assay.
This highly purified solution is suitable for 3-D matrix formation in cell culture. 3-D collagen gels imitate the in vivo cell physiology better than traditional 2D systems and has been proven successful for several cell types including cardian and corneal fibroblasts, depatic stellate cells, and neuroblastoma cells. Such 3-D gels are also useful in studies of mechanotransduction, cell signaling involving the transformation of mechanical signals into biochemical signals

Biochem/physiol Actions

Collagen type 1 is a versatile building material required for tissue elasticity and maintains the stability and strength in a variety of tissues. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissue.
In 3D environments, cell extensions can use integrins on cell surfaces to activate specific signaling pathways and integran-independent mechanical interactions resulting from the entanglement of matrix fibrils is possible.

General description

In mammals, collagen type 1 is a widely expressed fibrous protein. It constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix, and is also found in arterial walls, tendons, ligaments, skin, cornea, bone and dentin. Collagen structure possesses a triple helix polypeptide strand.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. As a heterodimer composed of two a1 chains and one a2 chains, it spontaneously forms a triple helix scaffold at neutral pH and 37°C.

Preparation Note

This product is prepared from type I bovine collagen purified and extracted from skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 6 mg/mL aqueous solution in 0.01 M HCl at a pH of 2.0.

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000511855
0000511855
0000472465
0000472465
0000474593

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Collagen in arterial walls: biomechanical aspects
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Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

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