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C1860

Sigma-Aldrich

Anti-Clathrin Heavy Chain antibody, Mouse monoclonal

clone TD.1, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD00162235
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TD.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 180-192 kDa

species reactivity

rat, human, bovine, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured rat adrenal pheochromocytoma PC-12 cells.

isotype

IgG1

UniProt accession no.

Q00610

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLTC(1213)
mouse ... Cltc(67300)
rat ... Cltc(54241)

General description

Clathrin, a three legged structure refer as triskelion is the main component of polygonal network and forms the coat of coated vesicles and coated pits. Clathrin-coated vesicles accounts for receptor mediated endocytosis at the plasma membrane and also play a crucial role in hormone secretion and mechanism of action. Monoclonal Anti-Clathrin Heavy Chain antibody can be used to study the mechanism of clathrin mediated membrane trafficking during hormonal stimulation and protein phosphorylation. Mouse monoclonal anti-clathrin heavy chain antibodies are react specifically with clathrin heavy chain CHC17 but do not cross react with muscle isoform CHC22.

Immunogen

globular N-terminal domain of bovine clathrin heavy chain.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Clathrin Heavy Chain antibody can be used in immunoblotting. It can also be used in western blotting to characterize clathrin heavy chain levels

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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David Rhainds et al.
Journal of cell science, 117(Pt 15), 3095-3105 (2004-07-01)
The scavenger receptor class B, type I (SR-BI) mediates cholesteryl esters (CE) selective uptake from low density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. In a number of tissues expressing caveolin, SR-BI is localized in caveolae. We show using detergent-free
P Narducci et al.
European journal of histochemistry : EJH, 54(1), e6-e6 (2010-04-01)
Bone is continuously repaired and remodelled through well-coordinated activity of osteoblasts that form new bone and osteoclasts, which resorb it. Osteoblasts synthesize and secrete two key molecules that are important for osteoclast differentiation, namely the ligand for the receptor of
I S Näthke et al.
Cell, 68(5), 899-910 (1992-03-06)
The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized.
Nicholas Rinkenberger et al.
Virology, 533, 59-67 (2019-05-22)
Zika virus (ZIKV) is an emerging pathogen with global health and economic impacts. ZIKV circulates as two major lineages, Asian or African. The Asian lineage has recently been associated with significant disease in humans. Numerous studies have revealed differences between
Guillaume A Castillon et al.
Traffic (Copenhagen, Denmark), 19(3), 215-228 (2018-01-21)
Recently, studies in animal models demonstrate potential roles for clathrin and AP1 in apical protein sorting in epithelial tissue. However, the precise functions of these proteins in apical protein transport remain unclear. Here, we reveal mistargeting of endogenous glycosyl phosphatidyl
View All Related Papers

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