C1650
Chitinase from Serratia marcescens
lyophilized powder, ≥10 units/g solid
Synonym(s):
Chitodextrinase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-578-7
MDL number:
form
lyophilized powder
specific activity
≥10 units/g solid
composition
Protein, 10-20% biuret
storage temp.
−20°C
Physical form
Lyophilized powder containing phosphate buffer salts
Other Notes
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay.
One new 1 hour unit = approx. 50 old 48 hour units.
One new 1 hour unit = approx. 50 old 48 hour units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Anna M Kielak et al.
Applied and environmental microbiology, 79(1), 263-272 (2012-10-30)
Chitin amendment is a promising soil management strategy that may enhance the suppressiveness of soil toward plant pathogens. However, we understand very little of the effects of added chitin, including the putative successions that take place in the degradative process.
Jogi Madhuprakash et al.
The Journal of biological chemistry, 287(53), 44619-44627 (2012-11-02)
We describe the improvement of transglycosylation (TG) by chitinase D from Serratia proteamaculans (SpChiD). The SpChiD produced a smaller quantity of TG products for up to 90 min with 2 mm chitotetraose as the substrate and subsequently produced only hydrolytic
WeiJuan Yang et al.
Biosensors & bioelectronics, 41, 820-826 (2012-11-06)
As one of the most destructive and widespread disease of rice, Magnaporthe grisea (M. grisea) has a significant negative impact on rice production. Therefore, it is significant to develop a method for sensitive and high-throughput detection of M. grisea in
Allergen bronchoprovocation and chitinases in allergic asthma.
G M Gauvreau
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 43(2), 149-151 (2013-01-22)
Wipa Suginta et al.
Bioscience, biotechnology, and biochemistry, 76(12), 2275-2281 (2012-12-12)
Three acidic residues in the DXDXE sequence motif are suggested to play a concerted role in the catalysis of Vibrio harveyi ChiA. An increase in the optimum pH of 0.8 units in mutant D313A/N indicates that Asp313 influences the pKa
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