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Merck
CN

C-14031

Normal Human Dermal Fibroblasts (NHDF)

Adult, 1 million cells per pellet

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About This Item

NACRES:
NA.81
UNSPSC Code:
41106514

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Product Name

Normal Human Dermal Fibroblasts (NHDF), Adult, 1 million cells per pellet

biological source

human dermis (adult)

packaging

pkg of 1,000,000 cells

morphology

(fibroblast)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

Related Categories

Analysis Note

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Application

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Biochem/physiol Actions

Skin

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Other Notes

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Preparation Note

Click here for more information.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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