Human Renal Epithelial Cells (HREpC)

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Kidney

Primary Human Renal Epithelial Cells (HREpC) are isolated from the adult kidney and are provided in a cryopreserved format. HREpC stain positive for cytokeratin.Kidneys have a very complex structure and contain diverse epithelia such as those in the convoluted tubules, the cortical collecting ducts, the calyxes, and the renal pelvis. The epithelial cells present in the distinct ducts and tubules differ in their morphology and physio-logical function. Consequently, HREpC are comprised of a heterogeneous population of epithelial cells. Important physiological processes like osmoregulation and excretion can be studied with HREpC in vitro.

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

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Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.