Skip to Content
Merck
CN
All Photos(1)

Key Documents

Safety Information

C-12241

Sigma-Aldrich

Human Pulmonary Artery Endothelial Cells (HPAEC)

500,000 cryopreserved cells

Synonym(s):

Human endothelial cells, Pulmonary artery endothelial cells

Sign Into View Organizational & Contract Pricing

Select a Size

50 μG
¥5,263.17

¥5,263.17

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order
All Photos(1)

Select a Size

Change View
50 μG
¥5,263.17

About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

¥5,263.17

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order

biological source

human pulmonary artery

packaging

pkg of 500,000 cells

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
102571025820273
Supelco

10462

Carbopack™ Adsorbent

Supelco

10257

Carbopack™ Adsorbent

Carbopack™ Adsorbent matrix Carbopack™ C, 80-100 mesh, bottle of 10 g

10258

Carbopack™ Adsorbent

Carbopack™ Adsorbent matrix Carbopack™ B, 60-80 mesh, bottle of 10 g

20273

Carbopack™ Adsorbent

surface area

~24 m2/g

surface area

~10 m2/g

surface area

~10 m2/g

surface area

~100 m2/g

pore size

~0 cm3/g macroporosity, ~0 cm3/g microporosity, ~0 cm3/g mesoporosity, ~0 Å pore diameter

pore size

~0 cm3/g macroporosity, ~0 cm3/g mesoporosity, ~0 cm3/g microporosity, ~0 Å pore diameter

pore size

~0 cm3/g macroporosity, ~0 cm3/g mesoporosity, ~0 cm3/g microporosity, ~0 Å pore diameter

pore size

~0 cm3/g macroporosity, ~0 cm3/g mesoporosity, ~0 cm3/g microporosity, ~0 Å pore diameter

form

granular

form

granular

form

granular

form

granular

matrix

Carbopack™ Y

matrix

Carbopack™ C

matrix

Carbopack™ C

matrix

Carbopack™ B

packaging

bottle of 10 g

packaging

bottle of 10 g

packaging

bottle of 10 g

packaging

bottle of 10 g

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Artery

Application

Primary Human Pulmonary Artery Endothelial Cells (HPAEC) are isolated from human pulmonary arteries (the main pulmonary artery, left and right branches) and are provided in a cryopreserved format. Since the pulmonary arteries carry blood from the heart to the lungs, they are the only arteries (other than umbilical arteries) that carry deoxygenated blood.

Quality

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

    It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

    If you need assistance, please contact Customer Support.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Protocols

    The Endothelial Cell Transwell Migration and Invasion Assay

    Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

    Related Content

    Endothelial Cell Tube Formation Angiogenesis Assay

    Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service