Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

C-12203

Sigma-Aldrich

Human Umbilical Vein Endothelial Cells (HUVEC)

Pooled, 500,000 cryopreserved cells

Synonym(s):

HUVEC cells

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human umbilical cord vein

packaging

pkg of 500,000 cells

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Vein

Application

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord. They are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. HUVEC are isolated from pooled donors (from up to four different umbilical cords) in standard Endothelial Cell Growth Medium and provided in a cryopreserved format. (Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.)

Quality

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P1Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Magdalena Surman et al.
International journal of molecular sciences, 23(22) (2022-11-27)
Bladder cancer is a malignancy that remains a therapeutic challenge and requires the identification of new biomarkers and mechanisms of progression. Several studies showed that extracellular vesicles promote angiogenesis, migration and metastasis, and inhibit apoptosis in bladder cancer. This effect
Sara Martinez de Lizarrondo et al.
Science advances, 8(28), eabm3596-eabm3596 (2022-07-21)
Molecular magnetic resonance imaging (MRI) holds great promise for diagnosis and therapeutic monitoring in a wide range of diseases. However, the low intrinsic sensitivity of MRI to detect exogenous contrast agents and the lack of biodegradable microprobes have prevented its
Beatriz Martínez-Burgo et al.
Immunology, 157(2), 173-184 (2019-04-24)
Leucocyte recruitment is critical during many acute and chronic inflammatory diseases. Chemokines are key mediators of leucocyte recruitment during the inflammatory response, by signalling through specific chemokine G-protein-coupled receptors (GPCRs). In addition, chemokines interact with cell-surface glycosaminoglycans (GAGs) to generate
Fabio Bertani et al.
International journal of molecular sciences, 22(24) (2021-12-25)
Cardiovascular diseases (CVDs), mainly ischemic heart disease (IHD) and stroke, are the leading cause of global mortality and major contributors to disability worldwide. Despite their heterogeneity, almost all CVDs share a common feature: the endothelial dysfunction. This is defined as
Barbara Clough et al.
PLoS pathogens, 12(11), e1006027-e1006027 (2016-11-23)
Toxoplasma gondii is the most common protozoan parasitic infection in man. Gamma interferon (IFNγ) activates haematopoietic and non-haematopoietic cells to kill the parasite and mediate host resistance. IFNγ-driven host resistance pathways and parasitic virulence factors are well described in mice

Articles

Introduction to Primary Cell Culture

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Protocols

The Endothelial Cell Transwell Migration and Invasion Assay

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Related Content

Endothelial Cell Tube Formation Angiogenesis Assay

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service