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C0984

Sigma-Aldrich

Creatine Kinase MB Fraction from human heart

≥70% (SDS-PAGE), liquid

Synonym(s):

CK MB Fraction

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About This Item

MDL number:
MFCD00151686
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

human heart

Quality Level

200

Assay

≥70% (SDS-PAGE)

form

liquid

concentration

≥0.5 mg/mL

technique(s)

IR spectroscopy: suitable

suitability

suitable for tissue

UniProt accession no.

P06732
P12277

application(s)

pharmaceutical

storage temp.

−20°C

Gene Information

human ... CKB(1152) , CKM(1158)

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General description

Research area: Cell Signaling
Creatine Kinase (CK) comprises M and B subunit and is a dimeric molecule. It is one among the isoenzymes of CK. The enzyme is majorly present in the myocardium.

Application

Creatine Kinase MB Fraction from human heart has been used:
  • as a diagnostic marker for acute myocardial infarction.
  • to identify overlapping absorbance peaks between blood and the cardiac biomarkers.
  • to study the phosphorylation pathway of dioxolane guanosine (DXG) in vivo, a potential treatment of HIV-1 infection.

Biochem/physiol Actions

Creatine Kinase (CK) catalyzes the formation of creatinine from creatine phosphate. The enzyme is associated with coronary performance. Elevated serum levels of CK-MB are prime indicators of myocardial cellular injury. It is regarded as a “gold standard” for myocardial infarction diagnosis. The levels of cardiac troponin I (cTnI) or cardiac troponin T also correlate to higher CK-MB) levels. CK-MB levels may also be elevated in chronic renal failure.

Components

Solution in 5 mM sodium succinate, 10 mM sodium chloride, 1 mM EDTA, 5 mM β-mercaptoethanol, 50% glycerol, pH 7.0.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000352053
0000352053
SLCP8404
SLCN0124
SLCL8978

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    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  5. What does the MB stand for in Product C0984, Creatine Kinase MB Fraction from human heart?

    Creatine Kinase is a dimer that exists as three isozymes: MM (muscle), MB (hybrid) and BB (brain).

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    Ask a Scientist here.

Terry M Welsh et al.
Annals of clinical and laboratory science, 32(1), 44-49 (2002-02-19)
The diagnosis of myocardial infarction (MI) is established in patients with chest pain and equivocal electrocardiogram changes by demonstrating a rise in blood levels of creatine kinase MB (CK-MB) and/or an increase in cardiac troponin I (cTnI) or cardiac troponin
Creatine Kinase
Clinical Methods: The History, Physical, and Laboratory Examinations (1990)
Anabolism of amdoxovir: phosphorylation of dioxolane guanosine and its 5?-phosphates by mammalian phosphotransferases
Feng JY, et al.
Biochemical Pharmacology, 68(9), 1879-1888 (2004)
J E Adams et al.
Clinical chemistry, 40(7 Pt 1), 1291-1295 (1994-07-01)
Although measurement of cardiac troponin I (cTnI) is, in some situations, more specific for detection of cardiac injury than is measurement of the MB isoenzyme of creatine kinase (MBCK), its sensitivity and specificity relative to MBCK for detection of myocardial
Development and preliminary validation of infrared spectroscopic device for transdermal assessment of elevated cardiac troponin
Titus J, et al.
Communications medicine, 2 (2022)
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Chromatograms

application for HPLC

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