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C0775

Sigma-Aldrich

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonym(s):

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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1 KG
CN¥1,202.23
5 KG
CN¥4,261.26

CN¥1,202.23

Estimated to ship onApril 28, 2025Details

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1 KG
CN¥1,202.23
5 KG
CN¥4,261.26

About This Item

Linear Formula:
C25H30N3Cl
CAS Number:
548-62-9
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
208-953-6
MDL number:
MFCD00011750
UNSPSC Code:
12171500
PubChem Substance ID:
57653980
NACRES:
NA.47

CN¥1,202.23

Estimated to ship onApril 28, 2025Details

Request a Bulk Order

Product Name

Crystal Violet, certified by the BSC

Agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

2.5 — 3.5 (20 °C)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

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1 of 4

This Item
193617193217A7330
乙酸铵 EMPLURA®

101115

乙酸铵

乙酸铵 EMPLURA®

193617

乙酸铵

乙酸铵 for analysis EMPARTA® ACS

193217

乙酸铵

乙酸铵 BioXtra, ≥98%

A7330

乙酸铵

assay

≥96.0% (alkalimetric)

assay

-

assay

-

assay

≥98%

solubility

1480 g/L

solubility

-

solubility

-

solubility

H2O: 1 M, clear, colorless

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

mp

114 °C

mp

-

mp

110-112 °C (dec.) (lit.)

mp

110-112 °C (dec.) (lit.)

density

1.17 g/cm3 at 20 °C

density

-

density

-

density

-

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability.[1] It has also been used for the staining of cells to study cell migration and invasion.[2]

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA.[3] Crystal violet is also used for the staining of bacteria in gram staining technique.[4] It is also used for the staining of plant chromosomes.[5] Crystal Violet also helps in colorimetric measurement of cell viability.[6]

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Signal Word

Danger

Hazard Statements

H302,H318,H351,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBS5375
    0000421139
    0000421139
    0000374312
    0000361587

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    Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
    Liao CJ, et al.
    Reproduction (Cambridge, England), 147, 179-179 (2013)
    Carson S and Robertson D
    Manipulation and Expression of Recombinant DNA (2005)
    A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
    Ishiyama M, et al.
    Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
    Cheesbrough M
    District Laboratory Practice in Tropical Countries (2006)
    Ping Jin et al.
    Cell death & disease, 10(8), 615-615 (2019-08-15)
    Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide. Development of novel chemotherapeutics is still required to enable successful treatment and improve survival for CRC patients. Here, we found that osimertinib (OSI) exhibits potent anti-CRC effects by
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