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C0662

Sigma-Aldrich

6-Carboxyfluorescein

~97% purity (HPLC), powder

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Synonym(s):
6-FAM, FLUOS
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
3301-79-9
Molecular Weight:
376.32
Beilstein:
54341
MDL number:
MFCD00036873
UNSPSC Code:
12171500
PubChem Substance ID:
24892266
NACRES:
NA.47

Quality Level

200

Assay

≥96% (HPLC)

form

powder

color

dark yellow to dark orange

mp

>300 °C (lit.)

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

λmax

492 nm at 0.1 N in NaOH

fluorescence

λex 492 nm; λem 517 nm (pH 8.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

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Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.
Fluorescent dye.

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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