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C0549

Sigma-Aldrich

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

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Synonym(s):
chloroform-isoamyl alcohol
UNSPSC Code:
12190000
NACRES:
NA.52

form

liquid

Quality Level

200

contains

0.6-1.0% ethanol as stabilizer

suitability

suitable for nucleic acid purification

application(s)

agriculture

General description

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.

Application

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
  • for a single DNA precipitation step for high-quality DNA extraction.
  • for protein extraction and RNAse treatment.
  • in phenolic oxidation to separate phenolics from the aqueous portion.
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Liver,Kidney, Respiratory system

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Regulatory Information

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A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
Journal of Separation Science, 46(2) (2017)
A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
Defective insulin-stimulated GLUT4 translocation in brown adipocytes induces systemic glucose homeostasis dysregulation independent of thermogenesis in female mice
Picatoste B, et al.
Molecular Metabolism, 53 (2021)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)
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