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C0481

Sigma-Aldrich

Protein Extraction Reagent Type 1

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About This Item

UNSPSC Code:
41116012
NACRES:
NA.56

form

solid

Quality Level

General description

Protein Extraction Reagent Type 1 is a highly effective protein solubilization and extraction reagent composed urea, Trizma® and CHAPS.

Application

Premixed and prequalified, Protein Extraction Reagent Type 1 is a highly effective protein solubilization and extraction reagent based on conventional extraction formulations. Reducing and alkylating reagents TBP (cat# T7567) and iodoacetamide (cat# A3221) are recommended for use with this reagent. Protein Extraction Reagent Type 1 is available as a component of the ProteoPrep® Sample Extraction Kit (PROTTOT).

Components

Contains urea, Trizma® and CHAPS

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Mallikarjun Gadwala et al.
Applied biochemistry and biotechnology, 169(4), 1459-1466 (2013-01-15)
The outstanding capability of two-dimensional gel electrophoresis in separating all types of proteins basically depends on the efficiency of sample preparation. Sample preparation is one of the most critical steps in two-dimensional gel electrophoresis. Unfortunately, due to severe solubility, resolution
Yuan Wang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(3), 919-933 (2018-07-24)
Chronic compression of the spinal cord causes the loss of motor neurons in the anterior horn, but the precise and extensive mechanism for the loss is not completely determined. Therefore, this study aims to explore the role of microRNA-494 (miR-494)
Xiaomeng Yi et al.
Experimental cell research, 383(2), 111454-111454 (2019-06-07)
Mesenchymal stem cells (MSCs) have been widely documented for their potential role in the treatment of various clinical disorders, including acute lung injury (ALI). ALI represents a clinical syndrome associated with histopathological diffuse alveolar damage. Recent evidence has demonstrated that
Yiming Deng et al.
Translational stroke research, 11(5), 950-966 (2020-01-31)
Ischemic stroke is associated with various physiological and pathological processes including neuronal apoptosis. Growth-arrest-specific transcript 5 (GAS5), a long non-coding RNA (lncRNA), has been recently reported to affect ischemic stroke-induced neuron apoptosis, while its mechanisms remain largely undefined. Through in silico
Andy Lo et al.
Analytica chimica acta, 788, 81-88 (2013-07-13)
Isotope labeling liquid chromatography-mass spectrometry (LC-MS) is a major analytical platform for quantitative proteome analysis. Incorporation of isotopes used to distinguish samples plays a critical role in the success of this strategy. In this work, we optimized and automated a

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