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BLD9604

GenElute 96 Well Blood Genomic DNA Purification Kit

4 x 96 well plate, sufficient for 4 96-well plate purifications

Synonym(s):

High Throughput Purification, Blood Genomic DNA

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About This Item

UNSPSC Code:
12352200
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Product Name

GenElute 96 Well Blood Genomic DNA Purification Kit, 4 x 96 well plate, sufficient for 4 96-well plate purifications

usage

sufficient for 4 96-well plate purifications

quality

4 x 96 well plate

storage temp.

15-25°C

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Application

The purified genomic DNA is ready for use in downstream applications such as:
  • PCR
  • restriction digest
  • Southern blots
  • cloning
  • enzymatic reactions

Biochem/physiol Actions

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.

Features and Benefits

  • Starting material: 200 μl fresh or frozen blood
  • Expected yield: 4 - 6 μg/well
  • Elution volume: 50 - 200 μl
  • Time required: 70 min/plate
  • Innovative wash plate minimizes risk of cross-contamination
  • Reduced risk of clogging
  • Complete processing possible at room temperature

General description

GenElute 96 Well Blood Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from whole blood, buffy coat, or cultured cells. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.

The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.

Other Notes

Please place blood binding plate at 4°C once you receive the kit. Other contents of the kit can be stored at room temp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

73.4 °F

flash_point_c

23 °C

Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can  be used as the "Square-well block" plate for the centrifugation protocol for Product BLD9604, GenElute 96 Well Blood Genomic DNA Purification Kit?

    Listed below are possible products that can be used as Square-well block plates with Product BLD9604, GenElute 96 Well Blood Genomic DNA. These are not provided with the kit. Product Z671371, Whatman® UNIPLATE microplates (Whatman, 7701-5200)Product CLS3960, Corning® 96 well storage system, sterile (Corning, 3960)Product CLS3961, Corning® 96 well storage system, non-sterile (Corning, 3961)

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