B9655
Anti-Avidin antibody, Mouse monoclonal, biotin conjugate
clone WC19.10, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-Avidin
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
WC19.10, monoclonal
form
buffered aqueous solution
technique(s)
direct ELISA: suitable
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.
Immunogen
Purified egg white avidin.
Application
Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women
Biochem/physiol Actions
Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays.
Physical form
Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
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Chapter 11 - (Strept)avidin?Biotin Systems
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Murine monoclonal anti-avidin antibodies enhance the sensitivity of avidin-biotin immunoassays and immunohistologic staining
Cassano WF, et al.
Journal of Immunological Methods, 117(2), 169-174 (1989)
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