Skip to Content
Merck
CN
All Photos(1)

Documents

B8781

Sigma-Aldrich

Restriction Enzyme Buffer SB

10 ×

Synonym(s):

Restriction Endonuclease Buffer

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204
NACRES:
NA.53

grade

for molecular biology

Quality Level

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

form

buffered aqueous solution

concentration

10 ×

storage temp.

−20°C

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Application

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+ C nucleotide content and secondary structure melting point
Bukovska P, et al.
Journal of Microbiological Methods, 82(3), 223-228 (2010)

Articles

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service