Anti-BrdU antibody, Mouse monoclonal

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

bromodeoxyuridine conjugated to KLH.

Anti-BrdU antibody has been used in:

• BrdU labeling
• immunohistochemistry
• immunostaining
• BrdU assay

Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:

• to detect 5-fluorouridine-labelled RNA
• to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
• against BrdU labelled rat retinal sections

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

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