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B8429

Sigma-Aldrich

Anti-Bax antibody, Mouse monoclonal

clone 6A7, purified from hybridoma cell culture

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Synonym(s):
Anti-BCL2L4
MDL number:
MFCD00282585
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6A7, monoclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa (and possibly a dimer of 42 kDa)

species reactivity

mouse, rat, human

concentration

2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 10-60 μg/mL using extract of human breast adenocarcinoma MCF-7 cells, activated with dexamethasone

isotype

IgG1

UniProt accession no.

Q07812

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BAX(581)
mouse ... Bax(12028)
rat ... Bax(24887)

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General description

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. The formation of bax homodimer accelerates apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis. Monoclonal anti-Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signalling. Mouse anti-Bax antibody reacts specifically with the epitope present within the amino acids 12-24 shared by bax from rat, mouse and human.
The B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) is mapped to human chromosome 19q13.33.

Immunogen

synthetic peptide corresponding to amino acids 12-24 of the human Bax sequence, conjugated to KLH.

Application

Anti-Bax antibody, Mouse monoclonal has been used in the western blotting detection in human retinal pigment epithelium cell lines, glioma, and breast cancer cell lines.
Monoclonal anti-Bax antibody can be used in immunocytochemistry (diluted 1: 1500) and western blot. It is also useful in microarray and immunoprecipitation.

Biochem/physiol Actions

Mutations in the B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) gene is implicated in colorectal cancer. BAX is associated with ovarian follicular dysfunction.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Sunny R Slone et al.
PloS one, 6(7), e21720-e21720 (2011-07-30)
Disruption of 14-3-3 function by alpha-synuclein has been implicated in Parkinson's disease. As 14-3-3s are important regulators of cell death pathways, disruption of 14-3-3s could result in the release of pro-apoptotic factors, such as Bax. We have previously shown that
Genetics of primary ovarian insufficiency: a review.
Fortuno C and Labarta E
Journal of Assisted Reproduction and Genetics, 31(12), 1573-1585 (2014)
Primary ovarian insufficiency.
De Vos M, et al.
Lancet, 376(9744), 911-921 (2010)
Xiaojian Zhang et al.
Molecular medicine reports, 10(5), 2549-2555 (2014-09-19)
Vitamin A (VA) has a number of important biological functions in human growth and development. Previous studies by our group demonstrated that the normal VA levels improved recovery of learning and memory function and decreased apoptosis in rats with hypoxic‑ischemic
Antitumor effects of arsenic disulfide on the viability, migratory ability, apoptosis and autophagy of breast cancer cells.
Zhao Y, et al.
Oncology Reports, 41(1), 27-42 (2019)
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