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B7632

Sigma-Aldrich

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

chromogenic, protease substrate, ≥98% (TLC), powder

Synonym(s):

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride

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100 FT
CN¥3,096.91

CN¥3,096.91

Available to ship onApril 14, 2025Details

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100 FT
CN¥3,096.91

About This Item

Empirical Formula (Hill Notation):
C33H40N8O6 · HCl
CAS Number:
54799-93-8
Molecular Weight:
681.18
Beilstein:
3027332
MDL number:
MFCD00038397
UNSPSC Code:
12352204
PubChem Substance ID:
24892011
NACRES:
NA.32

CN¥3,096.91

Available to ship onApril 14, 2025Details

Product Name

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Assay

≥98% (TLC)

form

powder

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

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Anti-NeuN Antibody, clone A60, Cy3 Conjugate clone A60, from mouse, CY3 conjugate

MAB377C3

Anti-NeuN Antibody, clone A60, Cy3 Conjugate

Anti-NeuN Antibody, clone A60, Alexa Fluor™ 555 Conjugate clone A60, from mouse, ALEXA FLUOR™ 555

MAB377A5

Anti-NeuN Antibody, clone A60, Alexa Fluor™ 555 Conjugate

Anti-NeuN (rabbit) Antibody, Cy3 Conjugate from rabbit, CY3 conjugate

ABN78C3

Anti-NeuN (rabbit) Antibody, Cy3 Conjugate

Anti-NeuN Antibody from chicken, purified by affinity chromatography

ABN91

Anti-NeuN Antibody

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

chicken

clone

A60, monoclonal

clone

A60, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

rat

species reactivity

rat, mouse

species reactivity

rat, mouse

species reactivity

mouse, rat

General description

N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.[1]

Application

  • N

  • -Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae[2]
  • for the thrombin, recombinant and native batroxobin from snake venom[3]
  • for fibrinolytic enzyme aprE2 in amidolytic activity assay[4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

A substrate for trypsin, thrombin and reptilase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5772
BCCD7744
BCCB9524
BCBV9946
BCBD4150V

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Substrates for determination of trypsin, thrombin and thrombin-like enzymes.
L Svendsen et al.
Folia haematologica (Leipzig, Germany : 1928), 98(4), 446-454 (1972-01-01)
The hydrolysis of N-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide and its use as a substrate for the assay of cathepsin B.
J Butterworth et al.
Analytical biochemistry, 106(1), 156-162 (1980-07-15)
Maxsuell Lucas Mendes Marques et al.
Marine drugs, 17(1) (2018-12-24)
In this study, sulfated polysaccharide-rich extracts were isolated from 22 tropical seaweeds (4 red, 11 brown, and 7 green) found in northeastern Brazil, and evaluated for the role of anticoagulant agents. Fifteen of the extracts showed anticoagulant activity, including all
I J Mackie et al.
Thrombosis research, 28(4), 499-507 (1982-11-15)
The Factor VIII content of Factor IX concentrates was investigated by agarose gel electrophoresis which removed the interfering effects of stabilisers and proteolytic enzyme inhibitors. Factor VIII coagulant activity (FVIII C) as measured by clotting and amidolytic methods correlated well
P C Ring et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 30(8), 1085-1096 (2000-08-10)
Fel d 1, an important allergen from domestic cats, is a significant cause of asthma. In addition to directly promoting IgE synthesis, other biological activities of allergens may contribute to either allergic sensitization or the magnitude of allergic effector responses.
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Protocols

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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