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About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
9E10, monoclonal
Application:
WB CL
Citations:
6
biological source
mouse
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
9E10, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
0.8 mg/mL
technique(s)
western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYC(4609)
General description
The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.
Immunogen
synthetic peptide of the human p62c-Myc protein.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
The antibody is suitable for immunoblotting.
Biochem/physiol Actions
Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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Illari Salvatori et al.
Journal of neurochemistry, 146(5), 585-597 (2018-05-21)
TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
Yan Wang et al.
PloS one, 7(12), e52404-e52404 (2012-12-29)
The neural cell adhesion molecule L1 plays important roles in neuronal migration and survival, neuritogenesis and synaptogenesis. L1 has also been found in tumors of different origins, with levels of L1 expression correlating positively with the metastatic potential of tumors.
Steven R Bates et al.
Applied physics letters, 95(7), 73705-73705 (2009-09-25)
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association
Ilja Demuth et al.
Oncogene, 23(53), 8611-8618 (2004-10-07)
DNA interstrand crosslinks (ICLs) are critical lesions for the mammalian cell since they affect both DNA strands and block transcription and replication. The repair of ICLs in the mammalian cell involves components of different repair pathways such as nucleotide-excision repair
Himari Ogino et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(12), 3181-3191 (2017-02-19)
The secreted glycoprotein Reelin regulates embryonic brain development and adult brain functions. It has been suggested that reduced Reelin activity contributes to the pathogenesis of several neuropsychiatric and neurodegenerative disorders, such as schizophrenia and Alzheimer's disease; however, noninvasive methods that
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