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Sigma-Aldrich

Anti-c-Myc−Biotin antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

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Synonym(s):
Anti-c-Myc, Monoclonal Anti-c-Myc−Biotin antibody produced in mouse
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.8 mg/mL

technique(s)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

UniProt accession no.

P01106

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

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General description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Specificity

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
The antibody is suitable for immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
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Applied physics letters, 95(7), 73705-73705 (2009-09-25)
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PloS one, 7(12), e52404-e52404 (2012-12-29)
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Romain Rouet et al.
Nature protocols, 7(2), 364-373 (2012-02-04)
Here we describe protocols for the expression of human antibody fragments in Escherichia coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview
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