B7405
Anti-Digoxin−Biotin antibody, Mouse monoclonal
clone DI-22, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DI-22, monoclonal
form
buffered aqueous solution
technique(s)
indirect ELISA: 1:10,000 using digoxin-labeled biomolecule
western blot: 1:500 using using digoxin-labeled biomolecule
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Immunogen
Digoxin-KLH.
Application
Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:
- in situ hybridization
- immunohistochemistry
- immunolocalization
Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.
Biochem/physiol Actions
Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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