B7405
Anti-Digoxin−Biotin antibody, Mouse monoclonal
clone DI-22, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
DI-22, monoclonal
Application:
ELISA (i), WB
Citations:
10
biological source
mouse
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DI-22, monoclonal
form
buffered aqueous solution
technique(s)
indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
Digoxin-KLH.
Application
Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:
- in situ hybridization
- immunohistochemistry
- immunolocalization
Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.
Biochem/physiol Actions
Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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F D Cuello-Carrión et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 47(6), 837-839 (1999-05-20)
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Differences in apoptotic status in the bovine placentome between spontaneous and induced parturition
HIrAyAmA H, et al.
Journal of Reproduction and Development (2012)
Mary C Bedard et al.
Cancers, 13(11) (2021-06-03)
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Il Soo Moon et al.
Journal of Korean medical science, 27(10), 1241-1247 (2012-10-24)
Long-term synaptic plasticity requires addition of new proteins at the synaptic site. The local protein synthesis at subsynaptic sites confers advantageous mechanisms that would regulate the protein composition in local domains on a moment-by-moment basis. However, our information on the
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Filliol A, et al.
Cell Death & Disease, 7(11), e2462-e2462 (2016)
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