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B7185

Sigma-Aldrich

Anti-Breast Cancer Resistance Protein antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-BCRP
MDL number:
MFCD03791618
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

mouse, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using heat-retrieved tissue sections from human term placenta by indirect immunoperoxidase staining of syncytiotrophoblasts.
western blot: 2.5-5 μg/mL using whole extract of human term placenta or mouse kidney and a chemiluminescent detection reagent

UniProt accession no.

Q9UNQ0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCG2(9429)
mouse ... Abcg2(26357)

General description

Anti-Breast Cancer Resistance Protein is developed in rabbit using as immunogen a synthetic peptide of human breast cancer resistance protein (BCRP). BCRP, also designated ABCG2, ABCP, placenta specific ATP-binding transporter and mitoxantrone-resistance protein (MXR), is a member of the ABCG subfamily of the large ATP binding cassette (ABC) transporter family of transmembrane proteins. BCRP is a half-transporter, with a single ATP binding cassette and transmembrane region. BCRP is normally expressed in human placental syncytiotrophoblasts, mammary ducts and lobules, endothelial cells and stem cells of different tissues.

Immunogen

synthetic peptide corresponding to amino acid residues 150-167 of human breast cancer resistance protein with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Breast Cancer Resistance Protein antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry
  • immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Up regulation of breast cancer resistance protein (BCRP) expression may be found in vivo and in vitro in both drugs treated and untreated tumors of different types. Breast cancer resistance protein transports various xenobiotics to the extracellular space through a process energized by ATP hydrolysis. In addition, to its role in protecting cells and tissues against xenobiotics especially in the intestine, liver, placenta and the blood-brain barrier, BCRP appears to be involved in hypoxic defense mechanisms and the protection and regulation of stem cells.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Breast cancer resistance protein in pharmacokinetics and drug-drug interactions
Xia CQ, et al.
Expert Opinion on Drug Metabolism & Toxicology, 1(4), 595-611 (2005)
Rare variants in the ABCG2 promoter modulate in vivo activity
Eclov RJ, et al.
Drug Metabolism and Disposition, 46(5), 636-642 (2018)
Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling
Nakanishi T, et al.
British Journal of Cancer, 102(5), 815-815 (2010)
Camilla Natasha Cederbye et al.
Scientific reports, 6, 26997-26997 (2016-06-04)
Overexpression of the ATP-dependent drug efflux pump ABCG2 is a major molecular mechanism of multidrug resistance in cancer and might be a predictive biomarker for drug response. Contradictory results have been reported for immunohistochemical studies of ABCG2 protein expression in
Arsenic-specific stem cell selection during malignant transformation
Tokar EJ, et al.
Journal of the National Cancer Institute, 638-649 (2010)

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