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B6125

Sigma-Aldrich

N-Benzoyl-L-tyrosine ethyl ester

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Synonym(s):
BTEE
Linear Formula:
4-(HO)C6H4CH2CH(NHCOC6H5)CO2C2H5
CAS Number:
3483-82-7
Molecular Weight:
313.35
Beilstein:
2223819
EC Number:
222-469-2
MDL number:
MFCD00002388
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24891896
NACRES:
NA.25

form

powder

Quality Level

200

mp

118-121 °C (lit.)

storage temp.

−20°C

SMILES string

CCOC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)c2ccccc2

InChI

1S/C18H19NO4/c1-2-23-18(22)16(12-13-8-10-15(20)11-9-13)19-17(21)14-6-4-3-5-7-14/h3-11,16,20H,2,12H2,1H3,(H,19,21)/t16-/m0/s1

InChI key

SRLROPAFMUDDRC-INIZCTEOSA-N

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General description

N-Benzoyl-L-tyrosine ethyl ester (BTEE) is a substrate which is completely resistant to trypsin and is required for the assay of chymotrypsin.

Application

N-Benzoyl-L-tyrosine ethyl ester has been used for measuring chymotrypsin activity.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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J Kantorski et al.
Journal of bioluminescence and chemiluminescence, 7(1), 37-45 (1992-01-01)
We have studied an indirect role of serine and thiol proteases in the activation of human neutrophils in vitro. Stimulation was evaluated using a chemiluminescence (CL) generation system. Receptor-dependent and receptor-independent stimuli were studied, e.g. opsonized zymosan, formyl-methionyl-leucyl-phenylalanine, platelet activating
Hydrolysis of polyesters by serine proteases.
Lim HA
Biotechnology Letters, 27, 459-464 (2005)
R Chatterjee et al.
Photochemistry and photobiology, 51(1), 91-97 (1990-01-01)
Skh:HR-1 hairless mice were irradiated chronically with sub-erythemal doses of UVB radiation, and a number of biochemical parameters in the skin were determined after 6, 12, 18, and 24 wk of exposure. The parameters measured were water, collagen, elastin, and
Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens.
Lee KW
British Poultry Science, 44, 450-450 (2003)
J M Mathys et al.
Journal of immunology (Baltimore, Md. : 1950), 164(3), 1588-1594 (2000-01-21)
Local TNF-alpha production in different organs may affect HIV replication and pathogenesis. Alveolar macrophages (AMs) obtained by bronchoalveolar lavage from asymptomatic HIV-seropositive and HIV-seronegative individuals did not spontaneously release TNF-alpha, but LPS stimulation of these cells significantly increased TNF-alpha production.
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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocols

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

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