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Merck
CN

B6032

BIS-TRIS hydrochloride

≥99.0% (titration)

Synonym(s):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5 · HCl
CAS Number:
Molecular Weight:
245.70
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
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Product Name

BIS-TRIS hydrochloride, ≥99.0% (titration)

InChI

1S/C8H19NO5.ClH/c10-3-1-9(2-4-11)8(5-12,6-13)7-14;/h10-14H,1-7H2;1H

SMILES string

Cl.OCCN(CCO)C(CO)(CO)CO

InChI key

VEYRVLHAMHQVTC-UHFFFAOYSA-N

assay

≥99.0% (titration)

form

crystalline powder

useful pH range

5.8-7.2

pKa 

6.5

solubility

water: 0.5 g/mL, clear, colorless to very faintly yellow

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Quality Level

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Application


  • Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions.: This research introduces the use of BIS-TRIS hydrochloride in Phos-tag SDS-PAGE systems to profile protein phosphorylation. The system operates under neutral pH conditions, making it suitable for analyzing proteins with diverse molecular weights. This method enhances the resolution of phosphorylated proteins, facilitating detailed phosphorylation studies crucial for understanding cellular signaling and protein function (Kinoshita et al., 2012).

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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