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B6008

Sigma-Aldrich

Ponceau BS

Dye content ~60 %, Powder

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Synonym(s):
Acid Red 66, Biebrich scarlet WS, Biebrich scarlet sodium salt
Empirical Formula (Hill Notation):
C22H14N4Na2O7S2
CAS Number:
4196-99-0
Molecular Weight:
556.48
Colour Index Number:
26905
Beilstein:
3883900
EC Number:
224-084-5
MDL number:
MFCD00003891
UNSPSC Code:
12171500
PubChem Substance ID:
24891888
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, ≥55%

color

red to brown

solubility

ethanol: 1 mg/mL
H2O: >30 mg/mL

εmax

≥19000 at 510 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3S([O-])(=O)=O)\N=N\c4ccc(cc4)S([O-])(=O)=O

InChI

1S/C22H16N4O7S2.2Na/c27-20-12-5-14-3-1-2-4-18(14)22(20)26-25-19-11-8-16(13-21(19)35(31,32)33)24-23-15-6-9-17(10-7-15)34(28,29)30;;/h1-13,27H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2/b24-23+,26-25+;;

InChI key

VVAVKBBTPWYADW-RVTJCSDESA-L

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Application

Ponceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.

Biochem/physiol Actions

Ponceau BS is used in Masson′s trichrome staining procedure, along with acid fuchsin, for the labeling of collagen and muscle red.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interleukin-4 has basophilic and eosinophilic cell growth-promoting activity on cord blood cells.
Favre C, et al.
Blood, 75, 67-67 (1990)
Shariff S and Kaler AK
Principles & Interpretation of Laboratory Practices in Surgical Pathology (2016)
Hong-Wen Gao et al.
Amino acids, 36(2), 251-260 (2008-03-28)
We studied the non-specific interactions of two azo compounds: biebrich scarlet (BS) and naphthochrome green (NG), with four model proteins: bovine serum albumin, ovalbumin, poly-L-lysine and hemoglobin by UV-VIS spectrometry, fluorophotometry and circular dichroism melting technique. The optimal acidities of
Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells.
Lee JW, et al.
Journal of Biomedical Materials Research Part A, 101, 1865-1865 (2013)
H R Allcock et al.
Biomaterials, 17(23), 2295-2302 (1996-12-01)
A new class of pH-sensitive hydrogels has been designed and synthesized. These are novel polyphosphazenes that bear various ratios of sodium oxybenzoate and methoxyethoxyethoxy side groups. These water-soluble macromolecules were cross-linked by 60Co gamma irradiation and the products were allowed
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