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Merck
CN

B5285

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt

≥98%, powder

Synonym(s):

BC-Indicator, X-GlcA, X-glucuronide

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About This Item

Empirical Formula (Hill Notation):
C14H12BrClNNaO7
CAS Number:
129541-41-9
Molecular Weight:
444.59
UNSPSC Code:
12352204
PubChem Substance ID:
24891832
NACRES:
NA.83
MDL number:
MFCD00135782

Key Documents

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Product Name

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, ≥98%

SMILES string

[Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1

InChI key

IBLSVGDGSKUDCT-CYRSAHDMSA-M

assay

≥98%

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

−20°C

Quality Level

200

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Application

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used:
  • as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay
  • to prepare X-Gluc or GUS staining solution
  • to confirm GUS expression in samples by X-gluc staining

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000462971
0000462970
0000462971
0000462970
0000363848

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Zhao Liu et al.
Bio-protocol, 7(14), e2446-e2446 (2017-07-20)
Determination of a gene expression in guard cells is essential for studying stomatal movements. GUS staining is one means of detecting the localization of a gene expression in guard cells. If a gene is specially expressed in guard cells, the
Agrobacterium tumefaciens-mediated Transformation of Walnut (Juglans regia)
Walawage SL, et al.
Plant Physiology (2014)
Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters
Patro S, et al.
Journal of Biotechnology, 163(3), 311- 317 (2013)
GUS Staining of Guard Cells to Identify Localised Guard Cell Gene Expression
Liu Z, et al.
Plant Physiology (2017)
Grégory Montiel et al.
Plants (Basel, Switzerland), 9(4) (2020-04-08)
Until recently, the roles of plant MADS-box genes have mainly been characterized during inflorescence and flower differentiation. In order to precise the roles of AGAMOUS-LIKE 12, one of the few MADS-box genes preferentially expressed in roots, we placed its cDNA
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