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B5285

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt

≥98%, powder

Synonym(s):

BC-Indicator, X-GlcA, X-glucuronide

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About This Item

Empirical Formula (Hill Notation):
C14H12BrClNNaO7
CAS Number:
129541-41-9
Molecular Weight:
444.59
MDL number:
MFCD00135782
UNSPSC Code:
12352204
PubChem Substance ID:
24891832
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, ≥98%

Quality Level

200

Assay

≥98%

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

[Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1

InChI key

IBLSVGDGSKUDCT-CYRSAHDMSA-M

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Application

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used:
  • as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay
  • to prepare X-Gluc or GUS staining solution
  • to confirm GUS expression in samples by X-gluc staining

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

related product

Product No.
Description
Pricing

907316

GlcA-ABP, 95%

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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