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B4436

Sigma-Aldrich

Anti-53BP1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-TP53BP1, Anti-p53 Binding Protein 1
MDL number:
MFCD09039188
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 345 kDa

species reactivity

mouse (predicted), rat (predicted), human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK 293-T cell lysates
microarray: suitable
western blot: 2-4 μg/mL using U2OS total cell extracts

UniProt accession no.

Q12888

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53BP1(7158)
mouse ... Trp53bp1(27223)
rat ... Tp53bp1(296099)

General description

Tumor protein p53 binding protein (T153BP1) is mapped to human chromosome 15q15.3. It has methylated histone-binding module and tandem tudor domains.

Immunogen

synthetic peptide corresponding to amino acids 1955-1972 of human 53BP1, conjugated to KLH via an N-terminal added cysteine residue. This sequence is conserved in mouse, and differs from rat by one amino acid

Application

Anti-53BP1 (C-terminal) antibody produced in rabbit has been used in western blotting and immunoprecipitation

Biochem/physiol Actions

Tumor protein p53 binding protein (T153BP1) has a close link to ataxia-telangiectasia mutated (ATM)-regulated events. 53BP1 becomes phosphorylated by ATM in a DNA damage-dependent manner suggesting that it participates in propagating the ATM signaling to its downstream effectors. T153BP1 also plays a key role in DNA repair by mediating nonhomologous end-joining (NHEJ) near double stranded breaks (DSBs). Fusion of T153BP1 with platelet-derived growth factor receptor beta (PDGFRβ) is implicated in a type of myeloproliferative disorder accompanied with eosinophilia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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p53-Binding protein 1 is fused to the platelet-derived growth factor receptor beta in a patient with at (5; 15)(q33; q22) and an imatinib-responsive eosinophilic myeloproliferative disorder
Grand FH, et al.
Cancer Research, 64(20), 7216-7219 (2004)
Michael T Perfetti et al.
ACS chemical biology, 10(4), 1072-1081 (2015-01-16)
Improving our understanding of the role of chromatin regulators in the initiation, development, and suppression of cancer and other devastating diseases is critical, as they are integral players in regulating DNA integrity and gene expression. Developing small molecule inhibitors for
Genomic landscape of liposarcoma
Kanojia D, et al.
Testing, 6(40), 42429-42429 (2015)
Role of 53BP1 oligomerization in regulating double-strand break repair
Lottersberger F, et al.
Proceedings of the National Academy of Sciences of the USA, 110(6), 2146-2151 (2013)
Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair
Botuyan MV, et al.
Cell, 127(7), 1361-1373 (2006)

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