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Safety Information

B4186

Sigma-Aldrich

Butyrylcholinesterase human

vial of ≥4 units

Synonym(s):

Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase

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About This Item

CAS Number:
9001-08-5
Enzyme Commission number:
3.1.1.8 (BRENDA, IUBMB)
EC Number:
232-579-2
MDL number:
MFCD00081455
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

200

specific activity

≥50 units/mg protein

packaging

vial of ≥4 units

UniProt accession no.

P06276

storage temp.

2-8°C

Gene Information

human ... BCHE(590)

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Application

BchE, from Sigma, has been used to determine butyrylcholinesterase inhibition by various test compounds .
Butyrylcholinesterase human has been used to study its role as a modulator of pathological features of Alzheimer′s disease (AD) in vivo.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. BChE is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates.
Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. It is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates. BChE hydrolyzes large esters of choline such as butyrylcholine and benzoylcholine. Selective inhibition of BChE activity can be used in the detection of organophosphates. BchE may be used to treat organophosphate toxicity. It is a critical target for Alzheimer′s disease (AD) therapy.

Unit Definition

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Analysis Note

Starting material individually donor tested and found negative for HIV I & II antibodies, Hepatitis B surfaceantigen, and Hepatitis C antibodies.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000323841
0000370239
0000336411
0000336411
0000370239

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What amount of enzyme is present in 1 vial of Product B4186, Butyrylcholinesterase from human serum?

    Each vial will contain at least 5 units. The vial content is given on the certificate of analysis of each lot. The vial may be substantially overpacked.

  6. How should Product B4186, Butyrylcholinesterase from human serum, be reconstituted?

    The enzyme can be reconstituted in 20 mM sodium phosphate buffer, pH 7.0, with 0.15 M sodium chloride.

  7. Are any buffer salts present in Product B4186, Butyrylcholinesterase from human serum?

    This product was lyophilized from 20 mM ammonium bicarbonate, which is a volatile buffer. So it would be expected to contain only traces of buffer salts.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The apolipoprotein E varepsilon4 allele plays pathological roles in AD through high protein expression and interaction with butyrylcholinesterase
Darreh-Shori T, et al.
Neurobiology of Aging, 32(7), 1236-1248 (2011)
Cholinesterases and engineered mutants for the detection of organophosphorus pesticide residues
Xu YL, et al.
Sensors, 18(12), 4281-4281 (2018)
Butyrylcholinesterase: an important new target in Alzheimer's disease therapy
Greig NH, et al.
International Psychogeriatrics / IPA, 14(S1), 77-91 (2002)
The key role of butyrylcholinesterase during neurogenesis and neural disorders: an antisense-5? butyrylcholinesterase-DNA study
Mack A and Robitzki A
Progress in Neurobiology, 60(6), 607-628 (2000)
A I Sacaan et al.
The Journal of pharmacology and experimental therapeutics, 280(1), 373-383 (1997-01-01)
Nicotine, the prototypical agonist for neuronal nicotinic acetylcholine receptors (NAChR), nonselectively activates NAChR limiting its use in elucidating the function of NAChR subtypes. SIB-1765F is a subtype selective NAChR agonist that displaces [3H]-nicotine binding with an IC50 of 4.6 nM
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