B3934
Biuret reagent
protein detection level 150-1,000 μg/mL
Synonym(s):
Micro Lowry, Onishi & Barr Modification
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About This Item
General description
Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.
Application
Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Proteins and Albumin
Roger LB
Laboratory Medicine, 45(1), e25?e41-e25?e41 (2014)
A simplified method of quantitating protein using the biuret and phenol reagents.
S T Ohnishi et al.
Analytical biochemistry, 86(1), 193-200 (1978-05-01)
Production of volatile fatty acids by fermentation of waste activated sludge pre-treated in full-scale thermal hydrolysis plants
Morgan-Sagastume F, et al.
Bioresource Technology, 102(3), 3089-3097 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B3934-110ML | 04061833429105 |